Monoaxial Mechanical Tests on Porcino Knee Ligaments / Ensaios mecânicos monoaxiais nos ligamentos do joelho porcino

Abstract The failure of ligament reconstruction has different risk factors, among which we can highlight the period before its incorporation, which is a mechanically vulnerable period. Loss of resistance over time is a characteristic of living tissues. Dissection with bone insertions of the cruciate ligaments of animal models is not described; however, it is essential for monoaxial assays to extract information from tests such as relaxation. The present work describes the dissection used for the generation of a test body for the performance of nondestructive tests to evaluate the mechanical behavior. We performed dissection of four porcino knee ligaments, proposing a dissection technique for the cruciate ligaments with bone inserts for comparison with collateral ligaments. The ligaments were submitted to relaxation tests and had strain gauges placed during the tests. The results showed viscoelastic behavior, validated by strain gauges and with a loss over time; with some ligaments presenting with losses of up to 20%, a factor to be considered in future studies. The present work dissected the four main ligaments of the knee demonstrating the posterior approach that allows maintaining their bone insertions and described the fixation for the monotonic uniaxial trials, besides being able to extract the viscoelastic behavior of the four ligaments of the knee, within the physiological limits of the knee.

Resumo A falha da reconstrução ligamentar tem diferentes fatores de risco, dentre os quais podemos destacar o período antes da sua incorporação, o qual configura um período mecânico vulnerável. A perda de resistência ao longo do tempo é uma característica dos tecidos vivos. A dissecção com as inserções ósseas dos ligamentos cruzados de modelos animais não é descrita; todavia, para os ensaios monoaxiais, é fundamental extrair as informações de ensaios como os de relaxação. O presente trabalho realiza a descrição da dissecção utilizada para a geração de corpo de prova para a realização de ensaios não destrutivos para avaliar o comportamento mecânico. Realizamos dissecção de quatro ligamentos de joelho porcino, propondo uma técnica de dissecção para os ligamentos cruzados com as inserções ósseas para comparação com os colaterais. Os ligamentos foram submetidos a testes de relaxação e foram colocadas strain gauges durante os testes. Os resultados mostraram comportamento viscoelástico, validado pelas strain gauges e com uma perda ao longo do tempo, sendo que, em alguns ligamentos, as perdas chegaram a até 20%, fator este a ser considerado em trabalhos futuros. O presente trabalho dissecou os quatro principais ligamentos do joelho, demonstrando a abordagem posterior que permite manter as suas inserções ósseas e descrevendo a fixação para os ensaios uniaxiais monotônicos, além de ter conseguido extrair o comportamento viscoelástico dos quatro ligamentos do joelho dentro dos limites fisiológicos do joelho.

Efecto de diferentes condiciones y modos de aplicación sobre la fuerza de unión de los adhesivos a la dentinaEffect of different conditions and modes of application on bond strength of adhesives to dentine / Effect of different conditions and modes of application on bond strength of adhesives to dentine

Objective: To evaluate the microtensile bond strength in different dentine conditions (etched-E, non-etched-N, dry-D and wet-W) of a multimode adhesive (Scotchbond Universal-SU, 3M/ESPE) and a total etching adhesive (Ambar-AB, FGM) using a sonic device (Smart Sonic Device-SD, FGM). Material and methods: In this in vitro study, ninety six sound extracted human molars were divided into 12 groups (n=8) according to different dentine conditions and adhesive systems. Enamel was removed and the middle dentine surfaces were polished. Each adhesive system was applied according to the different dentine conditions, and composite resin blocks were incrementally built up and stored for 24 hours. Specimens were sectioned into sticks and bond strength data were analyzed with the Kruskal-Wallis test and Mann-Whitney U tests. Results: No effects of sonic application and were observed. In general, AB showed lower results compared to the SU. E and N conditions did not statistically affect the bond strength of SU groups. Dry dentine presented statistically superior bond strength values when compared to wet dentine for SU/E/SD group. Conclusion: Adhesion of dry dentine with multimode adhesive system may be superior to wet dentine with sonic application. The modes of application had no influence in bond strength of studied adhesives.

Objetivo: Evaluar la resistencia de la unión microtensil en diferentes condiciones de dentina (grabado-E, sin grabado-N, seco-D y húmedo-W) de un adhesivo multimodo (Scotchbond Universal-SU, 3M/ESPE) y un adhesivo de grabado total (Ambar-AB, FGM) utilizando un dispositivo sónico (Smart Sonic Device-SD, FGM). Material y Métodos: En este estudio in vitro, noventa y seis molares humanos extraídos sanos se dividieron en 12 grupos (n=8) de acuerdo con diferentes condiciones de dentina y sistemas adhesivos. Se eliminó el esmalte y se pulieron las superficies centrales de la dentina. Cada sistema adhesivo se aplicó de acuerdo con las diferentes condiciones de dentina, y los bloques de resina compuesta se acumularon de forma incremental y se almacenaron durante 24h. Las muestras se seccionaron en barras y los datos de resistencia de la unión se analizaron con la prueba de Kruskal-Wallis y la prueba de U de Mann-Whitney. Resultado: No se observaron efectos de la aplicación sónica. En general, AB mostró resultados más bajos en comparación con el SU. Las condiciones E y N no afectaron estadísticamente la fuerza de unión de los grupos SU. La dentina seca presentó valores de fuerza de adhesión estadísticamente superiores en comparación con la dentina húmeda para el grupo SU/E/SD. Conclusión: La adhesión de la dentina seca con un sistema adhesivo multimodo puede ser superior a la dentina húmeda con aplicación sónica. Los modos de aplicación no tuvieron influencia en la resistencia de la unión de los adhesivos estudiados.

Microstructure and mechanical properties of hernia mesh / 国际生物医学工程杂志

Objective:The microstructure, tensile strength, and bursting strength of different brands of hernia meshes were compared and analyzed through experiments to evaluate the performance of different meshes.Methods:The balance and microscope were used to test the weight and microstructure of 15 common meshes and the tensile testing machine and burst testing machine were used to test the tensile and bursting properties of the mesh, and the mechanical properties of the mesh were analyzed.Results:The woven structures of the mesh are diamond, polygon and circle. The average weight of inguinal meshes is 0.08 mg/mm 2, and the average weight of abdominal wall hernia meshes is 0.18 mg/mm 2. The wire diameters of G3 - G6 meshes are larger, while the mesh opening ratio of G12 is lower. In the tensile performance test, it is known that G15 has the highest tensile strength, G12 and G14 have lower tensile strengths in lightweight meshes, and G1, G2, and G7 have lower tensile strengths in lightweight meshes. In the burst performance test, it is known that G3, G9, and G15 have the highest burst strength, while G12, G13, and G14 have the lowest burst strength in lightweight meshes. G1, G2, and G4 have the lowest burst strength in lightweight meshes. Conclusions:The mesh with a polygonal mesh and a large mesh opening ratio has better mechanical properties. The results of this study provide experimental evidence for optimizing hernia meshes, which is expected to provide better support for related research and applications.

Effect of S. mutans biofilm on the hybrid ceramic-resin cement bond strength assessed by different methods

Aim: The purpose of this study was to investigate the biofilm effect on the hybrid ceramic-resin cement bond strength (BS) by comparing two methods. Methods: Teeth were distributed into groups (n=5), according to the resin cement (Maxcem Elite-(MC) or NX3 Nexus-(NX)) and degradation method (24h or 7 days in distilled water; 7 or 30 days incubated with biofilm and 30 days in sterile media). Treated surfaces of Vita Enamic blocks (5x6x7mm) were luted to treated or no treated dentin surfaces and light-cured. After 24h, beams were obtained (1x1x10mm) and stored accordingly. The flexural bond strength (FBS) was assessed by four-point bending test. Additional beams were obtained from new teeth (n=5), stored for 24h or 7 days in distilled water, and submitted to a microtensile bond strength (µTBS) assay. Failure modes were determined by scanning electron microscopy (100X). The flexure strength of the cements (n=10) was assessed by a four-point bending test. Data were analyzed by 1 and 2-ways ANOVA, and Tukey's test (α=0.05). Results: There was no significant difference between the degradation methods for the FBS groups. For the µTBS, the significant difference was as follows: NX 7days > NX 24h > MC 7days = MC 24h. Failure mode was mainly adhesive and mixed, but with an increase of cohesive within cement and pre-failures for the MC groups assessed by µTBS. NX had better performance than MC, regardless of the method. Conclusions: The biofilm had no effect on the materials BS and FBS test was a useful method to evaluate BS of materials with poor performance

Effects of Different Storage Temperatures on the Properties of Nonlatex Orthodontic Modules

Objective: To evaluate and compare the effect of variation in storage temperatures and duration on a tensile load at failure of elastomeric modules. Methods: In total, 140 modules were used in the study, 20 of which were tested on day 0 as received from a company using a Universal testing machine for baseline estimation of tensile load at failure. The rest 120 modules were divided into 6 groups. Groups I, II, and III modules were stored at low (T1 = 1–5°C), moderate (T2 = 20–25°C) and high (T3 = 35–40°C) temperatures, respectively, for 6 months. Groups IV, V and VI modules were stored at temperatures T1, T2 and T3 for 1 year, respectively, and were tested for tensile load at failure. Results: The tensile load at failure for the control group was 21.588 ± 1.082 N and for 6?month interval at temperatures T1, T2 and T3 was 18.818 N ± 1.121 N, 17.841 N ± 1.334 N and 17.149 N ± 1.074 N, respectively, and for 1 year, it was 17.205 N ± 1.043 N, 16.836 N ± 0.487 N and 14.788 N ± 0.781 N, respectively. The tensile load at failure decreased significantly from 6 months to 1 year among each temperature group. Conclusions: Maximum force degradation was seen in modules at high temperature followed by medium temperature and low temperature at both 6 months and 1 year intervals, and tensile load at failure decreased significantly from 6 months to 1 year storage duration. These results conclude that the temperature and duration at which the samples were exposed during storage produce a significant change in the forces exerted by the modules.

Resistencia de cerámicas de disilicato de litio adheridas con dos adhesivos diferentes

Introducción: la cerámica de disilicato de litio es una excelente opción en rehabilitación, confeccionado con la técnica de inyección o maquinadas. El objetivo de este estudio fue evaluar la influencia de dos sistemas adhesivos sobre los valores de resistencia de unión en cerámicas de disilicato de litio inyectado y maquinado. Métodos: se utilizó discos de disilicato de litio, IPS e. Max CAD (Ivoclar Vivadent, Liechtenstein, Swiss), de 10mm de diámetro y 1mm de espesor embebidos en tubos de PVC. Se distribuyó en 4 grupos de trabajo, 2 en cerámica de litio inyectadas y 2 en maquinada, se aplicaron los adhesivos Palfique Bond y Tetric N-bond Universal según la distribución de grupos, luego se fotopolimerizó durante 10 segundos con lámpara luz led, se colocaron los tubos Tygon® para el relleno con resina fluida; una vez retirado los tubos Tygon®, se llevaron las muestras a una máquina de ensayo universal donde se realizó la prueba de micro cizallamiento. Resultados: los valores de resistencia de unión más significativos fueron encontrados en las cerámicas de disilicato de litio adherida con el adhesivo Palfique Bond y Tetric Bond teniendo una diferencia significativa (p= 0.001), los valores de resistencia de unión del adhesivo Palfique Bond y Tetric Bond en cerámica inyectada no tuvieron diferencia significativa. Conclusión: las cerámicas de disilicato de litio maquinadas presentan mejores valores de resistencia de unión al micro cizallamiento.

Introduction: lithium disilicate ceramics are an excellent choice in rehabilitation, made with the technique of injection or machining. The aim of this study was to evaluate the influence of two adhesive systems on the values of binding resistance in injected and machined lithium disilicate ceramics. Methods: lithium disilicate disks, IPS e. were used Max CAD (Ivoclar Vivadent, Liechtenstein, Swiss), 10mm in diameter and 1mm in thickness embedded in PVC tubes. Was distributed in 4 working groups, 2 in injected lithium ceramics and 2 in machined, Palfique Bond and Tetric N-bond Universal adhesives were applied according to the group distribution, then photopolymerized for 10 seconds with led light bulb, Tygon® tubes were placed for filling with fluid resin; once the Tygon® tubes were removed. The samples were taken to a universal testing machine where the micro-spraying test was performed. Results: the most significant binding resistance values were found in lithium disisilicate ceramics adhered with the Palfique Bond and Tetric Bond adhesive having a significant difference (p= 0,001), the bond strength values of the Palfique Bond and Tetric Bond adhesive in injected ceramic did not differ significantly. Conclusion: machined lithium disilicate ceramics have better values of binding resistance to micro-spraying.

Influence of diameter on mechanical behavior of morse taper narrow implants

Dental implants could give back function, esthetics and quality of life to patients. The correct choice of the implant, especially in borderline cases, is essential for a satisfactory result. Aim: Thus, the objective of this study was to evaluate the mechanical behavior of Morse taper implants with two different prosthetic interfaces. Methods: Twenty self-locking Morse taper implants, 2.9 mm in diameter (FAC), and 20 Morse taper implants, 3.5 mm in diameter (CM) were divided into two groups (n=10), and submitted to strength to failure test, optical microscopic evaluation of fracture, metallographic analysis of the alloy, finite element analysis (FEA) and strain gauge test. A Student's t test (α = 0.05) was made for a statistical analysis. Results: For the strength to failure test, a statistically difference was observed (p <0.001) between FAC (225.0 ± 19.8 N) and CM (397.3 ± 12.5 N). The optical microscopic evaluation demonstrated a fracture pattern that corroborated with FEA ́s results. The metallographic analysis determined that the implants of the FAC group have titanium-aluminum-vanadium alloy in their composition. In the strain gauge test, there was no statistical difference (p = 0.833) between CM (1064.8 ± 575.04 µS) and FAC (1002.2 ± 657.6 µS) groups. Conclusion: Based on the results obtained in this study, ultra-narrow implants (FAC) should ideally be restricted to areas with low masticatory effort

Bond strength of bulk-fill resin composites: the effect of cavity preparation and aging

Aim: Evaluating the resin-dentin bond strength of Class II conventional and bulk-fill composite restorations, using different cavity sizes before and after aging. Methods: Seventy-five human molars were distributed into groups according to the buccolingual width of the cavities, conservative (n=25) and extended (n=50). They were divided according to the restorative material: conventional (Z100/control group) or bulk-fill resin composites (Filtek Bulk Fill/FBF; Tetric N Ceram Bulk Fill/TNCBF; Filtek Bulk Fill Flow/FBFF; Surefill SDR flow/SDR). The restored teeth were sectioned on sticks (n=50 per restorative materials + width cavities group), half were stored in Water/Ethanol 75% for 30 days and the other half were submitted to the immediate microtensile bond strength (µTBS) test. Data were analyzed applying the Three-Way Analysis of Variance (ANOVA), Bonferroni test, test t, and Weibull analyses (p<0.05). Results: SDR and FBF presented lower µTBSvalues for extended preparation when compared to the conservative preparation, before aging. After aging, only for the FBFF, a decrease in the µTBSvalues was observed. Comparing the µTBSvalues, before and after aging, the SDR demonstrated lower µTBSvalues after aging when the conservative cavity was used. A decrease in the µTBSvalues was observed for the Z100, the FBF and, the FBFF, after aging, when the extended cavity was used. Conclusion: The effect of cavity preparation and aging on the resin-dentin of Class II is material dependent. Most of the bulk-fill resin composites evaluated presented a similar performance to the conventional resin composites for all the conditions of this study

Análisis biomecánico de la continuación prepatelar del cuádriceps / Biomechanical assessment of the prepatellar quadriceps continuation

OBJETIVO Determinar la resistencia máxima a la tracción (RMT) de la continuación prepatelar del cuádriceps (CPC).MATERIALES Y MÉTODOS Se realizó un estudio en cadáveres humanos. Fueron incluidos diez especímenes, en los cuales se utilizaron los tercios proximal y medial de la cortical anterior de la patela para evaluar las propiedades mecánicas de la CPC. En cada espécimen, se estudió un área de sección transversal de 0,2 cm2 (A1) y 1 cm2 (A2). Se aplicó una carga gradual para determinar la RMT.RESULTADOS La mediana de la RMT en el A1 fue de 232,56 N (rango: 141,23 N a 295,33 N) y en el A2 fue de 335,30 N (rango: 216,45 N a 371,40 N). El incremento en la TMR fue significativo entre las 2 áreas (p = 0,006).CONCLUSIÓN El ignificado clínico de este estudio es que la CPC es un tejido fuerte que puede servir de anclaje seguro para reconstrucciones alrededor de la patela. Un área relativamente pequeña tolera al menos 140 N y, a medida que crece el área, también aumenta la RMT.

OBJETIVE To determine the ultimate tensile strength (UTS) of the prepatellar quadriceps continuation (PQC). MATERIALS AND METHODS A human cadaveric study was performed. Ten fresh-frozen specimens were used. The proximal and medial thirds of the anterior cortex of the patella were used to assess the mechanical properties of the PQC. In each specimen, transverse section areas measuring 0.2 cm2 (A1) and 1 cm2 (A2) were studied. A gradual load was applied to determine the UTS. RESULTS The median UTS of A1 was of 232.56 N (range: 141.23 N to 295.33 N), and that of A2 was of 335.30 N (range: 216.45 N to 371.40 N). The increment in UTS was significant between the 2 areas (p » 0.006). CONCLUSION The clinical significance of the present study lies in the fact that it shows that the PQC is a strong tissue that can be a safe anchor for reconstruction around the patella. A relatively small area supports at least 140 N, and, as the area grows, the UTS increases as well.

Sistemas Adesivos Universais: um Panorama do Estado da Arte / Universal Adhesive Systems: a State-of-the-art Overview

Adhesive systems play a fundamental role in the adhesion of restorative materials to dental substrates. To make this adhesion more effective and long-lasting, these materials are in constant evolution, seeking to simplify the clinical steps and reduce the technique's sensitivity. The most recent generation of adhesives developed is the universal adhesives, which promise versatility and reduction of clinical steps. The aim of this study was to perform a literature review on universal adhesives and their characteristics. The literature review was performed by means of an electronic search in the Pubmed database. The literature shows that these adhesives chemically bond to tooth substrates and produce more stable and less hydrophilic dentin interfaces. However, some limitations exist when the use in selfetching mode is performed on enamel, and selective conditioning of this substrate is recommended. The use of these adhesives as a silane or primer in the cementation of glass-ceramics and metal alloys has also shown limitations. Nevertheless, in the cementation of zirconia-based ceramics and indirect composite resin-based restorations, the procedure can be simplified by the use of universal adhesives. Adhesive strength on dentin substrates under different conditioning modes varied between studies. As with any new material, long-term clinical evaluations are needed to demonstrate the efficacy of these universal adhesive agents, as reported in this literature review.

Os sistemas adesivos têm papel fundamental na adesão de materiais restauradores aos substratos dentários. Para que esta adesão seja cada vez mais eficaz e duradoura, estes materiais encontram-se em constante evolução buscando a simplificação de passos clínicos e diminuição da sensibilidade da técnica. A mais recente geração de adesivos desenvolvida é a dos adesivos universais, os quais prometem versatilidade e redução de passos clínicos. O objetivo deste trabalho foi realizar uma revisão de literatura sobre os adesivos universais e suas características. O levantamento bibliográfico foi realizado por meio de uma busca eletrônica na base de dados Pubmed. A literatura mostra que estes adesivos ligam-se quimicamente aos substratos dentários e produzem interfaces dentinárias mais estáveis e menos hidrofílicas. No entanto, algumas limitações existem quando o uso no modo autocondicionante é realizado em esmalte, sendo recomendado o condicionamento seletivo deste substrato. O uso destes adesivos como silano ou primer na cimentação de cerâmicas vítreas e ligas metálicas também demonstrou limitações. No entanto, na cimentação de cerâmicas a base de zircônia e nas restaurações indiretas a base de resina composta, o procedimento pode ser simplificado pelo uso dos adesivos universais. A resistência adesiva em susbstrato dentinário sob diferentes modos de condicionamento variou entre os estudos. Como qualquer novo material, avaliações clínicas de longo prazo são necessárias para demonstrar a eficácia destes agentes adesivos universais, conforme relatado nesta revisão de literatura.

Analysis of the mechanical behavior of the posterior cruciate ligament in a porcine model / Análise do comportamento mecânico do ligamento cruzado posterior em modelo suíno

ABSTRACT Introduction: The knee has shown a significant increase in the frequency of injury due to sports practice. This increase and the improvement of surgical techniques of ligament reconstruction have led to a greater indication of this treatment to achieve a function close to normal. Objective: To conduct a preliminary analysis of the ligament behavior. Methods: The study consisted of the validation of an anatomical model of the pig, in which five models were subjected to mechanical tests. The data on the loads of the in situ model and the strains of the posterior cruciate ligament were collected. Results: The analysis of the tensile load showed, at first, a nonlinear increase in stresses. Subsequently, the pig's knee showed a relatively linear intermediate response until failure around 1,200 N. Strain × time showed a response of the posterior cruciate ligament, which also has a relatively linear response. Conclusion: We observed a linear behavior in the range of 1,000 to 5,000 microstrains in the strain of the posterior cruciate ligament. We suggest further studies to understand knee ligaments regarding their behavior in their function. Level of Evidence IV, Biomechanical Study.

RESUMO Introdução: O joelho tem demonstrado um aumento significativo de frequência de lesão devido à prática esportiva. Esse aumento e a melhoria das técnicas cirúrgicas de reconstrução ligamentar têm levado a maior indicação desse tratamento com o objetivo de se atingir uma função próxima do normal. Objetivo: Realizar uma análise preliminar do comportamento ligamentar. Métodos: O estudo consistiu na validação de um modelo anatômico do porco, em que cinco modelos foram submetidos a ensaios mecânicos. Foram coletados os dados das cargas do modelo in situ e das deformações do ligamento cruzado posterior. Resultados: Na análise da carga trativa, foi observado que em um primeiro momento existe um aumento não linear das tensões. Em sequência, há uma resposta intermediária relativamente linear do joelho suíno até a falha em torno de 1.200 N. A deformação versus tempo mostrou uma resposta do ligamento cruzado posterior, que também possui uma resposta relativamente linear. Conclusão: Observou-se um comportamento linear na faixa de 1.000 até 5.000 microstrains na deformação do ligamento cruzado posterior. Sugerem-se novos estudos para a compreensão dos ligamentos do joelho quanto ao comportamento deles na sua função. Nível de Evidência IV, Estudo Mecânico.

Diametral tensile strength of conventional glass ionomer cements with modified powder ratio for uses in atraumatic restorative treatment / Resistência à tração diametral de cimentos de ionômero de vidro convencionais modificados na razão de pó para uso em tratamento restaurador atraumático

ABSTRACT Objective: To identify the diametral tensile strength of Brazilian brands of conventional glass ionomer cements (GIC) when modified powder ratio in their composition compared to each other, and with high viscosity glass ionomer cement for Atraumatic Restorative Treatment (ART). Methods: Experimental study with five groups (n=10 each) and specimens (2.5 mm in height and 5.0 mm in diameter), considering G1 (Maxxion R-MR), G2 (Vidrion R-VR) and G3 Vitro molar (VM-control) according to manufacturer recommending powder/liquid (1:1), G4 (MR) and G5 (VR) plus powder (2:1). The specimens were stored in an oven at 37 ± 1 ° C for 1 day. Diametral Tensile Test was performed by the Instron model 4444. Means differences were tested by Student´s T-Test (α = 5%). Results: There significant differences were in the means (standard deviation) of diametral tensile strength of MR (1:1) 4.24 (±1.47) and MR (2:1), 5.74 (±1.51) (p=0.039) and VR (1:1) 4.50 (±1.27) and VR (2:1) 6.20 (±1.89) (p=0.029). When comparing MR and VR (2:1) with MV (1:1) 4.82 (±1.17), VR 2: 1, 6.20 (±1.89) showed significant differences (p=0.0336). Conclusion: There was a significant increase in the means of diametral tensile strength of Brazilian brands conventional GICs when powder increment, approaching the performance observed by the high viscosity GIC.

RESUMO Objetivo: Identificar a resistência à tração diametral de marcas nacionais de cimentos de ionômero de vidro (CIV) convencionais quando acrescidos de pó em sua composição comparados entre si e com cimento de ionômero de vidro de alta viscosidade para Tratamento Restaurador Atraumático (TRA). Métodos: Pesquisa experimental com cinco grupos (n=10 cada) e confeccionados corpos de prova (2,5 mm de altura e 5,0 mm de diâmetro), considerando G1 (Maxxion R/FGM-MR), G2 (Vidrion R/SS White-VR) e G3 Vitro molar (DFL-VM-controle) manipulados segundo fabricante pó/líquido (1:1), G4 (MR) e G5 (VR) acrescidos de pó (2:1). Os espécimes foram armazenados em estufa a 37 ± 1° C por 1 dia. Teste de Tração Diametral foi realizado pelo equipamento Instron modelo 4444. As diferenças das médias foram testadas pelo Test T de Student (α=5%). Resultados: Foram encontradas diferenças significativas das médias (desvio-padrão) de resistência à tração diametral de MR (1:1) 4,24 (±1,47) e MR (2:1), 5,74(±1,51) (p=0,039) e VR (1:1) 4,50 (±1,27) e VR (2:1) 6,20(± 1,89) (p=0,029). Quando da comparação entre MR e VR (2:1) com VM (1:1) 4,82(±1,17), VR 2:1, 6,20 (±1,89) apresentou média superior e significante (p=0,0336). Conclusão: Observou-se aumento significativo nas médias de resistência mecânica à tração diametral dos CIVs convencionais nacionais a partir do incremento de pó, aproximando-se do desempenho observado pelo CIV de alta viscosidade.

Effect of Chelating Agents on Push-Out Bond Strength of NeoMTA Plus to Root Canal Dentin

ABSTRACT Objective To evaluate and compare the effect of 17% Ethylenediaminetetraacetic Acid (EDTA), 9% Etidronic acid (HEDP), and 7% Maleic acid (MA) on the push-out bond strength of NeoMTA Plus sealer to the coronal, middle, and apical thirds of root canal dentin. Material and Methods Forty single-rooted human maxillary central incisors were selected and decoronated to 12 mm long root fragments. Working length was established and root canals were then enlarged up to rotary Protaper F3. After each instrumentation, the root canal was irrigated with 2.5% NaOCl. For the final irrigation regimen, the specimens were divided into 4 groups (n=10) and treated with EDTA, HEDP, MA or Saline. Root canals were coated with NeoMTA Plus sealer, and obturation was done with single cone obturation technique. Subsequently, three horizontal sections were taken from the coronal, middle and apical thirds of each specimen, and POBS was measured using a universal testing machine. The type of bond failures was assessed under a stereomicroscope. Statistical analysis was done with One-way ANOVA with Tukey's Post hoc analysis. Results MA and EDTA showed the highest POBS. There was no significant difference in bond strength between MA and EDTA (p>0.05). HEDP and Saline showed lower POBS. Among all the four groups, the coronal third showed the highest values, followed by middle and apical thirds. Conclusion The type of chelating agent used during the root canal treatment significantly affects the bond strength of NeoMTA Plus to the root canal dentin.

The influence of prophylaxis with amino acid glycine powder and sodium bicarbonate jet on the bond strength of dental enamel / A influência da profilaxia com aminoácido glicina em pó e jato de bicarbonato de sódio na resistência de união do esmalte dentário

Aim: To compare the influence of prophylaxis with sodium bicarbonate and amino acid glycine powder on the bond strength of bovine teeth enamel and on the properties of two adhesive systems. Methods:Thirty-six extracted bovine incisors were randomly divided into six groups (n = 6) according to the prophylactic treatment received: no prophylactic treatment (NT), sodium bicarbonate powder (SB), and glycine powder (GL). Each group was subdivided into 2 groups based on what adhesive systems were used: conventional system (A) and universal system (B). Composite resin was applied on the buccal surface of the teeth in a block measurement 8x8x6 mm. The specimens were cut to obtain beams measuring 1.0 x 1.0 mm and were subjected to microtensile bond strength tests. Results were compared using two-way ANOVA (p ≤ 0.05). Results: The GLA group obtained the highest bond strength value for the conventional adhesive (18.97 MPa), but the GLB group obtained a lower strength value than the SBB group (GLB: 21.05 MPa and SBB: 22.29 MPa) (p < 0.05). Conclusions: Cleaning of the enamel surface increases the adhesive properties of restorative materials, and the bond strength was more effective in the group that received glycine prophylaxis and the conventional adhesive system.

Objetivo:Comparar a influência da profilaxia com bicarbonato de sódio e ClinproProphy ™ na resistência de união do esmalte de dentes bovinos e nas propriedades de dois sistemas adesivos.Métodos: Trinta e seis incisivos bovinos extraídos foram divididos aleatoriamente em 6 grupos (n = 6), cada um de acordo com o tratamento profilático recebido: nenhum tratamento profilático (NT), bicarbonato de sódio em pó (SB), glicina em pó (GL). Cada grupo foi subdividido em 2 grupos com base nos sistemas adesivos utilizados: sistema convencional (A) e sistema universal (B). A resina composta foi aplicada na superfície bucal dos dentes em um bloco de 8x8x6 mm. As amostras foram cortadas para obter blocos medindo 1,0 x 1,0 mm e submetidas a testes de resistência de união por microtração. Os resultados foram comparados usando o teste two-way ANOVA (p ≤ 0,05). Resultados: O grupo GLA obteve o maior valor de resistência de união para o adesivo convencional (18,97 MPa), mas o grupo GLB obteve um valor de resistência menor que o grupo SBB (GLB: 21,05 MPa e SBB: 22,29 MPa) (p < 0,05). Conclusão: A limpeza da superfície do esmalte aumenta as propriedades adesivas dos materiais restauradores, e a resistência adesiva foi mais eficaz no grupo que recebeu profilaxia com glicina e sistema adesivo convencional.

Effect of a novel radio-frequency atmospheric-pressure glow discharge plasma jet treatment on crosslinking of dentin collagen / 北京大学学报(医学版)

OBJECTIVE@#To investigate the effect of a noval radio-frequency atmospheric-pressure glow discharge (RF-APGD) plasma jet on crosslinking of dentin collagen.@*METHODS@#(1) Twenty intact third molars were collected. The middle dentin discs were prepared for each tooth by low-speed water-cooled Isomet saw, and then immersed in 10% (mass fraction) H3PO4 solution for 16 h to obtain fully demine-ralized dentin collagen. The twenty dentin discs were then randomly divided into five groups. The control group was untreated while the four experimental groups were treated by plasma jet with gas temperature of 4 ℃ for different times (20 s, 30 s, 40 s, and 50 s). The structure and crosslinking degree of dentin collagen were characterized by attenuated total reflection-Fourier transform infrared spectroscopy. The surface morphology of demineralized dentin was observed by scanning electron microscope, and the microstructure was observed by transmission electron microscope. (2) Fourty non-caries third molars were collected to prepare 5 g fine dentin powder, then completely demineralized with 10% H3PO4 solution. The control group was untreated, while the four experimental groups were treated by plasma jet for 20 s, 30 s, 40 s and 50 s. The crosslinking degree of each group was determined by ninhydrin colorimetric method. (3) Forty intact third molars were collected to obtain dentin strips. Only two central symmetrical dentin strips (nasty 80) were taken from each tooth and immersed in 10% H3PO4 solution for 16 h to obtain fully demineralized dentin collagen. Eighty dentine collagen fiber strips were randomly divided into five groups. The control group was untreated and the axial surfaces of dentin collagen fiber strips in the expe-rimental groups were treated with the plasma jet for 20 s, 30 s, 40 s and 50 s. The ultimate tensile strength of dentin was measured by universal mechanical machine.@*RESULTS@#(1) The surface morphology of demineralized dentin observed by scanning electron microscope showed that the network structure of collagen fibers on the surface of demineralized dentin treated with the plasma jet for 20 s, 30 s and 40 s could maintain the uncollapsed three-dimensional structure, and part of the microstructure was destroyed after plasma jet treated for 50 s. After being treated by plasma jet for 20 s, 30 s and 40 s, the three-dimensional structure was uncollapsed, and the typical periodic transverse pattern of natural type Ⅰ collagen fiber could be seen. The results of infrared spectra showed that the secondary conformation of dentin collagen fibers was the same as that of the control group after being treated with the plasma jet, and the intensity of amide band was significantly increased after being treated for 30 s and 40 s. (2) The results of ninhydrin crosslinking test showed that the crosslinking ratio of dentin collagen treated by plasma jet for 30 s and 40 s was the highest, and the difference was statistically significant (P < 0.05). (3) The results of dentin ultimate tensile strength showed that the control group was (1.67±0.24) MPa, and the plasma jet treated 20 s, 30 s, 40 s and 50 s groups were (4.21±0.15) MPa, (7.06±0.30) MPa, (7.32±0.27) MPa, and (6.87±0.17) MPa, which were significantly different from that of the control group (P < 0.05).@*CONCLUSION@#The novel RF-APGD plasma jet treatment can promote the crosslinking degree of demineralized dentin collagen and improve their ultimate tensile strength.

Resistencia a la fuerza de tracción de espigos de fibra de vidrio y anatomizados / Tensile bond strength of fiberglass and anatomized posts

Introducción: Es frecuente el desprendimiento de los espigos de fibra de vidrio por pérdida de adhesión cuando son sometidos a las fuerzas masticatorias. Objetivo: Comparar in vitro la resistencia a las fuerzas de tracción de espigos de fibra de vidrio y anatomizado con un mismo cemento. Métodos: Estudio in vitro, de tipo experimental. Se seleccionaron 20 premolares extraídos por motivos ortodónticos que fueron distribuidos aleatoriamente en dos grupos. Se les realizó el tratamiento de conductos por un especialista, la preparación fue manual y la técnica de obturación por condensación lateral. Los dientes fueron preparados para, en forma estándar, recibir el espigo de fibra de vidrio y anatomizados con resina. Para ambos grupos la cementación siguió el protocolo sugerido por el fabricante de Allcem Core® (cemento dual). Los especímenes fueron troquelados en acrílico de autocurado. Para luego ser sometido a fuerza de tracción vertical en la máquina de pruebas de materiales (Instrom©). Resultados: En el grupo de dientes con espigos anatomizados se obtuvo una media de 31 Kg/F, postes de fibra de vidrio 12,47 Kg/F. Según las pruebas de Shapiro-Wilk, las muestras presentaron distribución normal (p > 0,05). Se contrastó la hipótesis con la prueba de t de Student y se encontró diferencia estadística altamente significativas entre los grupos (p = 0,001) Conclusiones: Existe una mayor resistencia a la tracción vertical en los espigos anatomizados(AU)

Introduction: Fiberglass posts often fall off their position due to loss of adhesive capacity when they are subjected to masticatory force. Objective: Compare in vitro the tensile bond strength of fiberglass and anatomized posts cemented with the same adhesive material. Methods: An in vitro experimental study was conducted. A selection was made of 20 premolars extracted by orthodontic indication which were then randomly distributed into two groups. The premolars underwent root canal treatment by a specialist. The preparation was manual and sealing was performed by lateral condensation. The teeth were prepared in the standard manner to receive the fiberglass and resin-anatomized posts. In both groups' cementation followed the protocol suggested by the manufacturer of Allcem Core® (dual cement). The specimens were stamped in self-curing acrylic. Next, they were subjected to vertical tensile strength in an materials testing machine (Instrom©). Results: The mean values obtained were 31 kg/F for teeth with anatomized posts and 12.47 kg/F for fiberglass posts. According to Shapiro-Wilk tests, the samples exhibited a normal distribution (p > 0.05). The hypothesis was contrasted with Student's t test, obtaining a highly significant statistical difference between the groups (p = 0.001). Conclusions: Vertical tensile bond strength is greater in anatomized posts(AU)

Effect of different concentrations of green tea extract solutions on bonding durability of etchand- rinse adhesive system to caries affected dentin

Aim: The in vitro study evaluated the effect of different concentrations of green tea extract solution (GT) on the bonding durability of etch-and-rinse adhesive system to caries dentin affected (CAD). Methods: Dentinal surfaces of human third molars were polished and submitted to a microbiological caries induction protocol for 14 days. After removal of the infected dentin layer, the samples were randomly divided into 4 groups (n= 10), according to the concentration of GT solution applied in CAD, after acid etching: 0.05%; 0.2%; 2% and NT (no treatment ­ control). After application of a etch-and-rinse adhesive system (Adper Single Bond 2, 3M ESPE), composite resin restorations were performed on the dentin. After 24 hours, the resin-dentin blocks were sectioned 1mm2 specimens, which were subjected to the microtensile test immediately or after 6 months of storage in water. Data were submitted to two-way ANOVA for randomized blocks and Tukey test (α= 5%). Results: There was no effect of double interaction (p= 0.934). The application of 0.2% GT promoted a statistically significant increase in dentin bond strength values in comparison to the condition where GT was not used (p=0.012). There was a significant decrease of bond strength after 6 months of storage, regardless of dentin pretreatment (p = 0.007). The G test identified that there was no statistical difference regarding failure mode (p= 0.326). Conclusion: The concentration of 0.2% improved the bond strength of an etch-and-rinse adhesive system to caries affected dentin, however, none of dentin pretreatments could prevent the decrease in bond strength over time

Direct pedicle screw insertion pullout strength / Resistência ao arrancamento do parafuso pedicular de inserção direta

ABSTRACT Objective: Study the in vitro pullout strength of SpineGuard/Zavation Dynamic Surgical Guidance Z-Direct Screw (DSG Screw), a screw pedicle designed to be inserted using a direct insertion technique. Methods: DSG Screws of 5.5 mm and 6.5 mm were introduced into polyurethane blocks with a density of 10 PCF (0,16 g/cm3). According to the experimental group, screws were inserted without pilot hole, with pilot without tapping, undertapping and line-to-line tapping. Screw pullout tests were performed using a universal test machine after screw insertion into polyurethane blocks. Results: Screws inserted directly into the polyurethane blocks without pilot hole and tapping showed a statistically higher pullout strength. Insertion of the screw without tapping or with undertapping increases the pullout screw strength compared to line-to-line tapping. Conclusion: DSG Screw showed the highest pullout strength after its insertion without pilot hole and tapping. Level of Evidence V, Expert Opinion.

RESUMO Objetivo: Estudar a resistência ao arrancamento in vitro do parafuso de inserção direta da SpineGuard/Zavation (parafuso DSG), um parafuso pedicular projetado para ser inserido usando a técnica de inserção direta. Métodos: Parafusos DSG de 5,5 mm e 6,5 mm foram introduzidos em blocos de poliuretano com densidade de 10 PCF (0,16 g/cm3). De acordo com o grupo experimental, os parafusos foram inseridos sem orifício piloto, com orifício e sem macheamento e macheamento diâmetro inferior com mesma geometria. Os testes de resistência dos parafusos foram realizados usando uma máquina de teste universal após a inserção dos parafusos nos blocos de poliuretano. Resultados: Os parafusos inseridos diretamente nos blocos de poliuretano sem orifício piloto e sem macheamento apresentaram uma resistência de arrancamento com significância estatística maior. A inserção do parafuso sem macheamento ou com macheamento com diâmetro inferior apresenta maior resistência ao arrancamento em comparação com o macheamento do mesmo diâmetro. Conclusão: O parafuso DSG apresentou a maior resistência ao arrancamento após sua inserção sem orifício piloto e sem macheamento. Nível de Evidência V, Opinião do Especialista.

Evaluation of tensile strength after insertions andremovals of abutments on frictional Morse taper implants

ABSTRACT: This study aimed to evaluate possible changes in final retention after nine sequences of insertion and removal (SIR) of a frictional Morse taper implant/abutment system, evaluating the force required for dissociating this set between sequences, and verifying possible deformations in the implant heads. Ten implants, 13 mm long and 3.3 mm in diameter, were coupled to a universal mechanical testing machine. Ten anti-rotational abutments, 13 mm long and 3.5 mm in diameter, were connected to the implants parallel to the long axis, using an instrument called beat-connection, and subjected to tensile tests and SEM analysis. The results were analyzed using the Kruskal-Wallis test with Dunn's post-test, and the significance level was set at 5 %. There was no statistically significant difference in final retention among the nine SIRs evaluated. The force needed to uncouple the abutment from the implant increased as SIRs were performed on all ten implants, and an increase of 29.03 % was observed in the ninth SIR compared to the first SIR. After SEM analysis, no significant deformations, fractures, or cracks were observed in the implant heads.

RESUMEN: Este estudio tuvo como objetivo evaluar los posibles cambios en la retención final después de nueve secuencias de inserción y extracción (SIR) de un sistema de implante / pilar de cono de fricción Morse, evaluando la fuerza necesaria para disociar este conjunto entre secuencias y verificando posibles deformaciones en las cabezas de los implantes. Se acoplaron diez implantes, de 13 mm de largo y 3,3 mm de diámetro, a una máquina universal de ensayos mecánicos. Se conectaron a los implantes en paralelo al eje largo diez pilares antirrotacionales, de 13 mm de largo y 3,5 mm de diámetro, mediante un instrumento llamado beat-connection, y se sometieron a pruebas de tracción y análisis SEM. Los resultados se analizaron mediante la prueba de Kruskal-Wallis con la prueba posterior de Dunn, y el nivel de significancia se estableció en 5 %. No hubo diferencias estadísticamente significativas en la retención final entre los nueve SIR evaluados. La fuerza necesaria para desacoplar el pilar del implante aumentó a medida que se realizaban SIR en los diez implantes, y se observó un aumento del 29,03 % en el noveno SIR en comparación con el primer SIR. Después del análisis SEM, no se observaron deformaciones, fracturas o grietas significativas en las cabezas de los implantes.

The influence of ischemic preconditioning on neuromuscular performance / Influencia del preacondicionamiento isquémico en el desempeño neuromuscular / Influência do pré-condicionamento isquêmico no desempenho neuromuscular

ABSTRACT Introduction: Ischemic preconditioning (IPC) has been described in the literature as a resource capable of improving physical performance. Objective: The purpose of this randomized double-blind study was to evaluate the influence of IPC on the neuromuscular performance of trained individuals. Methods: Twenty-four (24) resistance training participants (6 of them women) with a mean age of 25.8 ± 4.6 years were selected and divided into two groups: the upper limb group (ULG) composed of 12 individuals (4 women) and the lower limb group (LLG) composed of 12 individuals (2 women). The maximum repetitions test was applied in the bench press for the ULG and in the 45° leg press for the LLG, with 50% of the one-repetition maximum under control, placebo and IPC conditions, at a random interval of 72 hours between tests. The IPC was applied four hours before the tests by means of an analog sphygmomanometer cuff inflated to 220 mmHg on the arm for the ULG and on the thigh for LLG, with three cycles of five minutes each of ischemia and reperfusion, alternating between the right and left sides. For the placebo, the cuff was inflated to 40 mmHg without causing ischemia. The significance level for the Wilcoxon test was p <0.017, due to the Bonferroni correction. The effect size (ES) was also analyzed. Results: With IPC, the ULG performed 34.8 ± 4.8 repetitions, representing an improvement of 11.29% (IPC vs. control, ES = 0.68 and p = 0.002) and the LLG performed 40.5 ± 15.7 repetitions, representing an improvement of 37.47% (IPC vs. control, ES = 0.84 and p = 0.002). No significant improvements were observed for the placebo in either group. Conclusion: Our data showed that IPC positively influenced neuromuscular performance of both the upper and lower limbs. Level of evidence II; Therapeutic studies investigating the results of treatment (Prospectived comparative studye).

RESUMEN Introducción: El preacondicionamiento isquémico (PCI) ha sido descrito en la literatura como un recurso capaz de mejorar el desempeño físico. Objetivo: El objetivo de este estudio aleatorio doble ciego fue evaluar la influencia del PCI en el desempeño neuromuscular de individuos entrenados. Métodos: Fueron seleccionados 24 individuos (6 mujeres) con promedio de edad de 25,8 ± 4,6 años, practicantes de entrenamiento resistido, divididos en dos grupos, siendo un grupo de miembros superiores (GMS) compuesto por 12 individuos (4 mujeres) y grupo de miembros inferiores (GMI) compuesto por 12 individuos (2 mujeres). El test de repeticiones máximas fue aplicado en el ejercicio de supino para el GMS y en el leg press 45° para el GMI con 50% de una repetición máxima, en las condiciones de control, placebo y PCI, de forma aleatoria con intervalo de 72 horas entre los tests. El PCI fue aplicado cuatro horas antes de los tests mediante un manguito de esfigmomanómetro analógico inflado a 220 mmHg en el brazo para el GMS y en el muslo para el GMI, siendo tres ciclos de cinco minutos de isquemia y cinco minutos de reperfusión, alternando los lados derecho e izquierdo. Para el placebo, el manguito quedó en 40 mmHg, sin provocar isquemia. El nivel de significancia del test de Wilcoxon fue p <0,017, debido a la corrección de Bonferroni. El tamaño del efecto (TE) también fue analizado. Resultados: Con el PCI, el GMS realizó 34,8 ± 4,8 repeticiones, representando mejora de 11,29% (PCI vs control, TE = 0,68 y p = 0,002) y el GMI realizó 40,5 ± 15,7 repeticiones, representando mejora de 37,47% (PCI vs control, TE = 0,84 y p = 0,002). Con el placebo, ambos grupos no mostraron mejora. Conclusión: Nuestros datos mostraron que el PCI influenció positivamente el desempeño neuromuscular tanto de miembros superiores como inferiores. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento (estudio comparativo prospectivo).

RESUMO Introdução: O pré-condicionamento isquêmico (PCI) tem sido descrito na literatura como um recurso capaz de melhorar o desempenho físico. Objetivo: O objetivo deste estudo randomizado duplo cego foi avaliar a influência do PCI no desempenho neuromuscular de indivíduos treinados. Métodos: Foram selecionados 24 indivíduos (6 mulheres) com média de idade de 25,8 ± 4,6 anos, praticantes de treinamento resistido, divididos em dois grupos, sendo um grupo de membros superiores (GMS), composto por 12 indivíduos (4 mulheres) e grupo de membros inferiores (GMI), composto por 12 indivíduos (2 mulheres). O teste de repetições máximas foi aplicado no exercício de supino para o GMS e no leg press 45° para o GMI com 50% de uma repetição máxima, nas condições de controle, placebo e PCI, de forma aleatória com intervalo de 72 horas entre os testes. O PCI foi aplicado quatro horas antes dos testes por meio de um manguito de esfigmomanômetro analógico inflado a 220 mmHg no braço para o GMS e na coxa para o GMI, sendo três ciclos de cinco minutos de isquemia e cinco minutos de reperfusão, alternando os lados direito e esquerdo. Para o placebo, o manguito ficou em 40 mmHg, sem provocar isquemia. O nível de significância do teste de Wilcoxon foi de p < 0,017, devido à correção de Bonferroni. O tamanho do efeito (TE) também foi analisado. Resultados: Com o PCI, o GMS realizou 34,8 ± 4,8 repetições, representando melhora de 11,29% (PCI vs. controle, TE = 0,68 e p = 0,002) e o GMI realizou 40,5 ± 15,7 repetições, representando melhora de 37,47% (PCI vs. controle, TE = 0,84 e p = 0,002). Com o placebo, ambos os grupos não apresentaram melhora. Conclusão: Nossos dados mostraram que o PCI influenciou positivamente o desempenho neuromuscular tanto de membros superiores quanto inferiores. Nível de evidência II; Estudos terapêuticos-Investigação dos resultados do tratamento (Estudo prospectivo comparativo).